Staphylokinase derivatives

Details Staphylokinase derivatives Staphylokinase derivatives

1995-01-11

1997-12-09

Jagannathan, Vasu S.

Drug, bio-affecting and body treating compositions

Enzyme or coenzyme containing

Hydrolases

435220, A61K 3848, C12N 952

Patent

active

056957548

ABSTRACT:
A method for producing the derivatives of the invention by preparing a DNA fragment comprising at least the part of the coding sequence of staphylokinase that provides for its biological activity; performing in vitro site-directed mutagenesis on the DNA fragment to replace one or more codons for wild-type amino acids by a codon for another amino acid; cloning the mutated DNA fragment in a suitable vector; transforming or transfecting a suitable host cell with the vector; and culturing the host cell under conditions suitable for expressing the DNA fragment. Preferably the DNA fragment is a 466 bp EcoRI-HINDIII fragment of the plasmid pMEX602SAK, the in vitro site-directed mutagenesis is performed by an oligonucleotide-directed mutagenesis system using the plasmid pMa/c and the repair deficient E. coli strain WK6MutS, and the mutated DNA fragment is cloned in E. coli strain-WK6. The invention also relates to pharmaceutical compositions comprising at least one of the staphylokinase derivatives according to the invention together with a suitable excipient, for treatment of arterial thrombosis.

REFERENCES:
Schlott, et al. Biochimica et Biophysica Acta 1204:235-242 (1994).

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