Staphylococcus aureus rsbU-1

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues

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C07K 1431

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active

061110787

ABSTRACT:
rsbU-1 polypeptides and DNA (RNA) encoding such rsbU-1 and a procedure for producing such polypeptides by recombinant techniques is disclosed. Also disclosed are methods for utilizing such rsbU-1 for the treatment of infection, particularly bacterial infections. Antagonists against such rsbU-1 and their use as a therapeutic to treat infections, particularly bacterial infections are also disclosed. Also disclosed are diagnostic assays for detecting diseases related to the presence of rsbU-1 nucleic acid sequences and the polypeptides in a host. Also disclosed are diagnostic assays for detecting polynucleotides encoding SigB operon and for detecting the polypeptide in a host.

REFERENCES:
Wu, et al., "Sigma-B, a Putative Operon Encoding Alternate Sigma Factor of Staphylococcus aureus RNA Polymerase: Molecular Cloning and DNA Sequencing" Journal of Bacteriology, vol. 178, No. 20, pp. 6036-6042, Oct. 1996.
Kullik, et al., "The Alternative Sigma Factor .sigma..sup.B in Staphylococcus aureus: Regulation of the sigB Operon in Response to Growth Phase and Heat Shock", Arch Microbiol, vol. 167, pp. 151-159, (1997).
Benjamini, et al., Immunology--A Short Course, Third Edition, Published by Wiley-Liss, (1996).
Davis, et al., "Direct Gene Transfer in Skeletal Muscle: Plasmid DNA-based Immunization Against the Hepatitis B Virus Surface Antigen", Vaccine, vol. 12, No. 16, pp. 1503-1509, (1994).
Voelker, et al, "The Yeast Two-Hybrid System Detects Interactions Between Bacillus subtillis .sigma..sup.B Regulators", Journal of Bacteriology, vol. 178, No. 23, pp. 7020-7023, Dec., 1996.
Sigma Chemical Company catalogue (1993) p. 1761.
Stuart Orkin, M.D. and Arno G. Motulsky, M.D., "Report and Recommendations of the Panel to Assess the NIH Investment in Research on Gene Theraphy" Dec. 7, 1995.

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