Staphylococcal fibronectin receptor, monoclonal antibodies there

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

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536 14, 536 172, 536127, 536128, C07H 100, C07H 1522, C07H 1702, C07H 106

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050345150

ABSTRACT:
A purified fibronectin receptor polysaccharide derived from Staphylococcus aureus is useful as an antigen for diagnostic tests and the preparation of monoclonal antibodies. The fibronectin receptor polysaccharide is prepared by gently removing expresed material, including the polysaccharide, from the cell surfaces of the S. aureus without killing the cells followed by purification. Monoclonal antibodies directed against the polysaccharide can be used in methods of preventing or treating S. aureus infections by administering the monoclonal antibodies to animals.

REFERENCES:
patent: 4197290 (1980-04-01), Yoshida
Proctor, Richard A., The Staphylococcal Fibronectin Receptor: Evidence for Its Importance in Invasive Infections, Reviews of Infectious Diseases, vol. 9, Supplement 4, Jul.-Aug. 1987, pp. S335-S340.
Proctor, Richard A. et al., Fibronectin Mediates Attachment of Staphylococcus aureus to Human Neutrophils, Blood, vol. 59, No. 4, Apr. 1982, pp. 681-687.
Proctor, Richard A. et al., Subinhibitory Concentrations of Antibiotics Alter Fibronectin Binding to Staphylococcus aureus, Antimicrobial Agents and Chemotherapy, vol. 24, No. 5, Nov. 1983, pp. 823-826.
Proctor, Richard A. et al., Effects of Subinhibitory Concentrations of Antibiotics on Staphylococcus aureus Interactions with Fibronectin, Journal of Antimicrobial Chemotherapy (1983) 12, Suppl. C. 85-95.
Mosher, Deane F. et al., Fibronectin: Role in Inflammation, Advances in Inflammation Research, vol. 2, 1981, pp. 187-207.
Mosher, Deane F., et al., Binding and Factor XIII.sub.a --Mediated Cross-Linking of a 27-Kilodalton Fragment of Fibronectin to Staphylococcus aureus, Science, vol. 209, Aug. 1980, pp. 927-929.
Proctor, Richard A. et al., Fibronectin Binding to Staphylococcus aureus, Journal of Biological Chemistry, vol. 257, No. 24, Dec. 1982, pp. 14788-14794.
Proctor, Richard A. et al., Fibronectin-induced Agglutination of Staphylococcus aureus Correlates with Invasiveness, The Journal of Laboratory and Clinical Medicine, vol. 104, No. 4, Oct. 1984, pp. 455-469.
Mitchell, J. Nelles, et al., Reactivity of Type-Specific Monoclonal Antibodies with Staphylococcus aureus Clinical Isolates and Purified Capsular Polysaccharide, Infection and Immunity, vol. 49, No. 1, Jul. 1985, pp. 14-18.
Hochkeppel, H. K. et al, Serotyping and Electron Microscopy Studies of Staphylococcus aureus Clinical Isolates with Monoclonal Antibodies to Capsular Polysaccharide Types 5 and 8, Journal of Clinical Microbiology, vol. 25, No. 3, Mar. 1987, pp. 526-530.
Huycke, Mark M. et al., Specific Inhibition of Fibronectin (FN) Binding to a Staphylococcus aureus (Sa) FH Receptor (FNR).

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