Standard platelet samples and method for preparation thereof

Details Standard platelet samples and method for preparation thereof Standard platelet samples and method for preparation thereof

1992-04-22

1995-10-10

Saunders, David

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 724, 435 792, 435963, 435967, 436 10, 436 63, G01N 33531

Patent

active

054570280

ABSTRACT:
A method for determining the activation level of platelets and other types of blood cells that undergo activation by ELISA and other immunoassay techniques includes the step of reacting a sample containing the blood cells in a liquid phase with an excess quantity of an activation-specific primary antibody prior to allowing the cells in the sample to bind to a solid surface. This differs from typical ELISA procedures, wherein the cells are bound to the surface of a plastic test tube or microtiter plate prior to addition of the primary antibody. It has been discovered according to the invention that binding the cells to a surface prior to reaction with the primary antibody changes the activation level of the cells, making the assay less accurate.

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Berman et al., Methods in Enzymology 169:314, 1989.
The Role of Fibrinogen Aa Chains in ADP-Induced Platelet Aggregation in the Presence . . . Amrani et al., pp. 919-924 (1988)-Blood, vol. 72, No. 3.
Shattil et al J. Biol. Chem. vol. 260, No. 20 pp. 11107-11114, 1985.
M. Wilchek et al, Immunology Today, 5, 39-43, 1984.

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