Standard or control material for glysocylated or total hemoglobi

Chemistry: analytical and immunological testing – Composition for standardization – calibration – simulation,... – Protein or peptide standard or control

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436 13, 436 16, 436 17, 436 66, 436 67, 424101, 435 2, 435188, C09K 300, G01N 3372, G01N 3396

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044657749

ABSTRACT:
A control material for use in the determination of glycosylated and total hemoglobin, and a method of preparing the same, are disclosed. The control is prepared by the steps of hemolyzing red blood cells, preferably in the presence of a detergent, followed by removal of stroma and lipids from the hemoglobin with an organic solvent such as toluene in the presence of a polyhydroxy compound such as sucrose. A hemoglobin-rich fraction is separated from the resulting mixture and dialyzed against a solution of a polyhydroxy compound. The dialysate may be lyophilized to provide a stable, dry product suitable for reconstitution.

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