Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...
Patent
1993-10-04
1997-06-17
Benzion, Gary
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide alters fat, fatty oil, ester-type wax, or...
4351723, 4351721, 435419, 435414, 935 36, 935 35, 935 67, 47 58, 47DIG1, 536 241, 536 232, 536 236, C12N 1500, C12N 1582, A01H 106, A01M 400
Patent
active
056399480
DESCRIPTION:
BRIEF SUMMARY
This invention relates to promoters isolated from rice which can provide gene expression predominantly or specifically in stamen cells of a plant, particularly a monocotyledonous plant, and thereby provide little or no gene expression in other parts of the plant that are not involved in the production of fertile pollen. The promoters are useful in the production of transformed plants, in which a gene is to be expressed at least predominantly, and preferably specifically, in the stamen cells, preferably in the anther cells. The promoters are especially useful in the production of male-sterile plants and male fertility-restorer plants as described in European patent applications ("EPA") 89401194.9 and 90402281.1, respectively (which are incorporated herein by reference), particularly in the production of hybrids of monocotyledonous plants, such as corn, rice or wheat.
SUMMARY OF THE INVENTION
In accordance with this invention are provided male flower-specific cDNA sequences isolated from rice comprising the sequences: SEQ ID no. 1, SEQ ID no. 2, SEQ ID no. 3, SEQ ID no. 4 and SEQ ID no. 5 shown in the Sequence Listing. Also in accordance with this invention are provided the stamen-specific, preferably anther-specific, particularly tapetum-specific, promoters of the rice genes corresponding to such cDNA sequences, particularly the promoter PT72 upstream from nucleotide 2846 of SEQ ID no. 6, the promoter PT42 upstream from nucleotide 1809 of SEQ ID no. 7, and the promoter PE1 upstream from nucleotide 2264 of SEQ ID no. 8 shown in the Sequence Listing. These promoters can each be used in a foreign DNA sequence, preferably a foreign chimaeric DNA sequence, which contains a structural gene, preferably a male-sterility DNA or a male fertility-restorer DNA, under the transcriptional control of one of the promoters and which can be used to transform the nuclear genome of a cell of a plant, particularly a monocotyledonous plant. Further in accordance with this invention are provided: the male-sterile plant or male fertility-restorer plant which can be regenerated from such a cell transformed with the foreign DNA sequence of this invention; the cells, cell cultures and seeds of such a plant; and the male fertility-restored plant and its seeds resulting from crossing such male-sterile and male fertility-restorer plants.
DETAILED DESCRIPTION OF THE INVENTION
In accordance with this invention, a male-sterile plant or a male fertility-restorer plant can be produced from a single cell of a plant by transforming the plant cell in a known manner to stably insert, into its nuclear genome, the foreign DNA sequence of this invention. The foreign DNA sequence comprises at least one male-sterility DNA or male fertility-restorer DNA that is: under the control of, and fused in frame at its upstream (i.e., 5') end to, one of the stamen-specific, preferably anther-specific, particularly tapetum-specific, promoters of this invention; and fused at its downstream (i.e., 3') end to suitable transcription termination (or regulation) signals, including a polyadenylation signal. Thereby, the RNA and/or protein or polypeptide, encoded by the male-sterility or fertility-restorer DNA is produced or overproduced at least predominantly, preferably exclusively, in stamen cells of the plant. The foreign DNA sequence can also comprise at least one marker DNA that: encodes a RNA and/or protein or polypeptide which, when present at least in a specific tissue or specific cells of the plant, renders the plant easily separable or distinguishable from other plants which do not contain such RNA and/or protein or polypeptide at least in the specific tissue or specific cells; is under the control of, and is fused at its 5' end to, a second promoter which is capable of directing expression of the marker DNA at least in the specific tissue or specific cells; and is fused at its 3' end to suitable transcription termination signals, including a polyadenylation signal. The marker DNA is preferably in the same genetic locus as the male-sterility or fertilit
REFERENCES:
Tchang et al. "Phospholipid Transfer Protein: Full-Length cDNA and Amino Acid Sequence in Maize". vol. 263, No. 32 pp. 16849-16855 Nov. 15, 1988.
Komari Toshihiko
Michiels Frank
Morioka Sinji
Scheirlinck Trees
Benzion Gary
Plant Genetic Systems N.V.
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