Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...
Patent
1993-08-05
1996-12-31
Veitenheimer, Erich E.
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide alters fat, fatty oil, ester-type wax, or...
800250, 800DIG56, 4351723, 4353201, 4352404, 536 221, 536 236, 536 241, 536 245, 536 243, 935 6, 935 30, 935 35, A01H 100, A01H 500, A01H 510, C12N 1510, C12N 1529
Patent
active
055896103
DESCRIPTION:
BRIEF SUMMARY
FIELD OF THE INVENTION
This invention relates to promoters isolated from corn which can provide gene expression predominantly or specifically in stamen cells of a plant, particularly a monocotyledonous plant, and thereby provide little or no gene expression in other parts of the plant that are not involved in the production of fertile pollen. The promoters are useful in the production of transformed plants, in which a gene is to be expressed at least predominantly, and preferably specifically, in the stamen cells, preferably in the anther cells. The promoters are especially useful in the production of male-sterile plants and male fertility-restorer plants as described in European patent applications ("EPA") 89401194.9 and 90402281.1, respectively (which are incorporated herein by reference), particularly in the production of hybrids of monocotyledonous plants, such as corn, rice or wheat.
SUMMARY OF THE INVENTION
In accordance with this invention are provided: male flower-specific cDNA sequences isolated from corn comprising the sequences, SEQ ID no. 1 and SEQ ID no. 2, shown in the sequence listing. Also in accordance with this invention are provided stamen-specific, preferably anther-specific, promoters of the corn genes corresponding to such cDNA sequences, particularly the promoter which controls the expression of the genomic coding sequence corresponding to the cDNA of SEQ ID no. 2 and which is contained within the sequence of nucleotides 1 to 1179 of SEQ ID no. 3 (the "CA55 promoter" or "PCA55"). Each of such promoters can be used in a foreign DNA sequence, preferably a foreign chimaeric DNA sequence, which contains a structural gene, preferably a male-sterility DNA or a male fertility-restorer DNA, under the transcriptional control of the promoter and which can be used to transform the nuclear genome of a cell of a plant, particularly a monocotyledonous plant. Further in accordance with this invention are provided: the male-sterile plant or male fertility-restorer plant which can be regenerated from such a cell transformed with the foreign DNA sequence of this invention; the transformed cell, itself; a culture of such a transformed cell; seeds of such a regenerated plant and its progeny; and a fertility-restored plant and its seeds resulting from crossing such male-sterile and male fertility-restorer plants.
DETAILED DESCRIPTION OF THE INVENTION
In accordance with this invention, a male-sterile plant or a male fertility-restorer plant can be produced from a single cell of a plant by transforming the plant cell in a known manner to stably insert, into its nuclear genome, the foreign DNA sequence of this invention. The foreign DNA sequence comprises at least one male-sterility DNA or male fertility-restorer DNA that is: under the control of, and fused in frame at its upstream (i.e., 5') end to, one of the stamen-specific, preferably anther-specific, particularly tapetum-specific, promoters of this invention, such as the promoter and optionally the leader sequence of SEQ ID no. 3; and fused at its downstream (i.e., 3') end to suitable transcription termination (or regulation) signals, including a polyadenylation signal. Thereby, the RNA and/or protein or polypeptide, encoded by the male-sterility or male fertility-restorer DNA, is produced or overproduced at least predominantly, preferably exclusively, in stamen cells of the plant. The foreign DNA sequence can also comprise at least one marker DNA that: encodes a RNA and/or protein or polypeptide which, when present at least in a specific tissue or specific cells of the plant, renders the plant easily separable or distinguishable from other plants which do not contain such RNA and/or protein or polypeptide at least in the specific tissue or specific cells; is under the control of, and is fused at its 5' end to, a second promoter which is capable of directing expression of the marker DNA at least in the specific tissue or specific cells; and is fused at its 3' end to suitable transcription termination signals, including a polyadenylation signal.
REFERENCES:
patent: 5086169 (1992-02-01), Mascarenhas
Koltunow et al., "Different Temporal and Spatial Gene Expression Patterns Occur During Anther Development", Plant Cell, 2, 1201-1224 (1990).
Mariani et al., "Genetic Destruction of Tapetal Cells Results in the Production of Male Sterile Plants", J. Cell. Biochem., Supplement15A, 21 (1991).
Rieger et al. 1976. Glossary of Genetics and Cytogenetics. Springer-Verlag. pp. 446+516.
Gordon-Kamm et al. 1990, The Plant Cell. 2:603-618.
Quattrocchio et al. 1990, Plant Molecular Biology. 15:81-83.
De Beuckeleer Marc
Gossele Veronique
Herdies Lydia
Mariani Celestina
Plant Genetic Systems N.V.
Veitenheimer Erich E.
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