Staining, de-staining and quantification of proteins by coomassi

Details Staining, de-staining and quantification of proteins by coomassi Staining, de-staining and quantification of proteins by coomassi

1997-07-28

1999-07-13

Beisner, William H.

Chemistry: electrical and wave energy

Apparatus

Electrophoretic or electro-osmotic apparatus

204462, G01N 2726, G01N 27447

Patent

active

059221860

ABSTRACT:
The present invention presents a novel method for both staining electrophoresis gels, as well as removing the background dye during de-staining. This novel and simple pad-based technique minimizes solvent consumption, is environmentally friendly, and does not leave any solid residues on the gel. Furthermore, a novel method for the quantitative and qualitative determination of protein concentration in solution is also presented. This method is simple, easy-to-use and has an easily visible color change from clear to blue in the presence of protein.

REFERENCES:
patent: 4021324 (1977-05-01), Delony et al.
patent: 4357174 (1982-11-01), Rushbrook et al.
patent: 4750506 (1988-06-01), Olexa

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