Stain for acid-fast bacilli

Details Stain for acid-fast bacilli Stain for acid-fast bacilli

1987-04-06

1989-08-15

Naff, David M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

4352521, 4352531, 435863, C12Q 104, C12N 120, C12R 132

Patent

active

048574597

ABSTRACT:
Acid-fast bacilli are stained with a primary stain that does not require heating and then with a decolorizing counter-stain that does not require a separate acid or alcohol decolorization step. The primary stain includes basic fuchsin, ethyl alcohol, isopropyl alcohol, phenol and water. The counter-stain includes methylene blue, ethyl alcohol, potassium hydroxide, glycerol, glacial acetic acid, polyvinylpyrrolidone and water.

REFERENCES:
patent: 4489158 (1984-12-01), Straus
patent: 4575484 (1986-03-01), Straus
patent: 4692412 (1987-09-01), Livingston et al.

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