Drug – bio-affecting and body treating compositions – Radionuclide or intended radionuclide containing; adjuvant... – Molecular bilayer structure
Patent
1985-03-04
1987-05-19
Hollrah, Glennon H.
Drug, bio-affecting and body treating compositions
Radionuclide or intended radionuclide containing; adjuvant...
Molecular bilayer structure
424 3, G01N 3100, G01N 3348
Patent
active
046666990
ABSTRACT:
A stain-fixative composition, for use in the microscopic identification of enteric protozoa and other parasites in fecal specimens, contains Ponceau S Stain, Chlorazol Fast Pink BK Stain, Trypan Blue Stain, dimethyl sulfoxide and a fixative component. The fixative component may be either a polyvinyl alcohol fixative or may be a mixture of isopropanol, acetic acid, liquified phenol, distilled water and optional glutaraldehyde. The stain-fixative composition is simple to prepare, has a long shelf life at ambient conditions, is easy to use and is suitable for preparing formalin or tap water concentrates, wet mount slides, or permanent smears prepared with gelatin-glycerol or resinous mounting media. In addition, the stain-fixative composition is free from the toxic mercury compounds often found in prior art staining reagents. The stain-fixative composition gives superior fixation and resolution, yet its use requires a minimum of time and attention in the laboratory. The method of staining fecal specimens for the identification of enteric parasites is also disclosed.
REFERENCES:
M. M. Brooke, D. Sc., "Polyvinyl Alcohol-Fixative as a Preservative and Adhesive for Protozoa in Dysenteric Stools and Other Liquid Materials, 1949, pp. 1554-1560.
R. B. Borrows, "Improved Preparation of Polyvinyl Alcohol-H.sub.g Cl.sub.2 Fixative used for Fecal Smears," (1967) pp. 93-56 (Wellcome Research Labs).
B. B. Gardner et al., "Comparison of Direct Wet Mount and Trichrome Staining Techniques for Detecting Entamoeba Species Trophozoites in Stools," (1980), vol. 12, No. 5, pp. 656-658.
L. Shore Garcia et al., "Diagnostic Parasitology Clinical Laboratory Manual," (1975), Chap. 5, pp. 16-20.
W. B. Wheatly, "A Rapid Staining Procedure for Intestinal Amoebae and Flagellates" (1951), pp. 990-991.
J. H. Rampey, Jr., "Modified DMSO-Trichrome Technic for Staining Intestinal Protozoa in Fresh Fecal Smears," (1968), vol. 49, No. 4, p. 611.
D. J. Flournoy et al., "Rapid Trichrome Stain," (1982), Journal of Clinical Microbiology, vol. 16, No. 3, pp. 573-574.
Manual of Clinical Microbiology, 3rd ed., American Society for Microbiology, Washington, D.C. (1980), p. 685.
H. J. Conn's Biological Stains, Ninth ed., R. D. Lillie, M.D. (1977), pp. 165, 639.
McGraw-Hill Encyclopedia of Science and Technology, Parasitology, pp. 628-630, vol. 9.
Allegheny-Singer Research Institute
Dinner D.
Hollrah Glennon H.
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