Staged immobilized amyloglucosidase and immobilized glucose isom

Details Staged immobilized amyloglucosidase and immobilized glucose isom Staged immobilized amyloglucosidase and immobilized glucose isom

1984-06-11

1986-04-15

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C12P 1924

Patent

active

045828032

ABSTRACT:
The limitation of an immobilized amyloglucosidase in hydrolyzing thinned starch to afford not more than about 93% glucose with isomaltose levels above about 1.5% can be overcome in a process for converting thinned starch to fructose using four closely coupled reactor stages. The first stage is a saccharification reactor using amyloglucosidase which converts thinned starch to a product containing from 50% to 85% glucose. This product is used in a first stage isomerization reactor, the effluent from which is sent to another saccharification reactor using immobilized amyloglucosidase where hydrolysis is continued until no more than about 6% disaccharides and higher oligosaccharides are present. Where this effluent is used as a feedstock for further conversion of glucose to fructose, it is operationally equivalent to a feedstock containing at least 94% glucose but with isomaltose levels under about 1.5%.

REFERENCES:
patent: 4382121 (1983-05-01), Rohrbach et al.

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