Stably human transfected rodent fibroblast cell line expressing

Details Stably human transfected rodent fibroblast cell line expressing Stably human transfected rodent fibroblast cell line expressing

1995-04-05

1998-02-17

Ulm, John

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Rodent cell, per se

435 72, 435 691, 4352523, 4353201, 530350, C12N 510

Patent

active

057190577

ABSTRACT:
The present invention relates to a stably co-transfected eukaryotic cell line capable of expressing a GABA-A receptor, particularly a human GABA-A receptor, which receptor comprises at least one alpha, one beta and one gamma subunit; to the cloning of novel cDNA sequences encoding the .alpha.-2, .alpha.-3, .alpha.-5, .alpha.-6 and .beta.-2 subunits of the human GABA-A receptor; and to the use of the cell line in designing and developing GABA-A receptor subtype-selective medicaments.

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Kleingoor et al. "Inverse but not full benzodiazepine agonists modulate recombinant alpha-6, beta-2, gamma-2 GABA-A receptors . . . ", Neuroscience Letters, vol. 130, pp. 169-172 (1991).
Moss et al., "Cloned GABA receptors are maintained in a stable cell line: allosteric and channel properties", Eur. Jour. of Pharma., vol. 189, pp. 77-88 (1990).
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