Stable yeast 2 .mu.m vector

Details Stable yeast 2 .mu.m vector Stable yeast 2 .mu.m vector

1994-09-19

1997-06-10

Fleisher, Mindy

Chemistry: molecular biology and microbiology

Vector, per se

435 691, 435 692, 435 711, 435171, 4351721, 4351723, 4352551, 935 6, 935 9, 935 22, 935 33, 935 57, 935 59, 935 60, 935 61, 935 66, 935 69, C12N 1500, C12N 116, C12P 2106, C12P 102

Patent

active

056375042

ABSTRACT:
A 2 .mu.m plasmid vector for transforming yeast, especially brewing yeast, much which comprises a DNA sequence allowing propagation of the plasmid in bacteria, two copies of the 74 base pair FLP recombination site of the 2 .mu.m plasmid in direct orientation and a DNA sequence coding for a protein or peptide of interest. The plasmid is so constructed that in yeast the bacterial DNA sequence is between the two recombination sites which are in direct orientation and is spontaneously lost following recombination. A "gene of interest" is preferable inserted at the SnaBI site.

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