Stable two-part reagent for the measurement of creatine kinase a

Details Stable two-part reagent for the measurement of creatine kinase a Stable two-part reagent for the measurement of creatine kinase a

1996-01-16

1998-02-10

Saucier, Sandra E.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

435194, C12Q 150

Patent

active

057167974

ABSTRACT:
A stable two-part liquid reagent composition for measuring creatine kinase activity contains, in the first part, adenosine 5'-adiphosphate, kexokinase or glucokinse, nicotinamide adenine dinucleotide (phosphate), glucose-6-phosphate dehydrogenase, and thioglycerol, 2-mercaptoethanol, or 2-mercaptoethanesulfonic acid or a salt thereof; and contains, the second part, creatine phosphate at a pH of 7.5 to 10.0. Glucose and magnesium ions can be included in either part or in both parts. The two-part composition is stable even after being stored for 3 months at 10.degree. C.

REFERENCES:
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