Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – Involving particle-mediated transfecion
Reexamination Certificate
1999-07-30
2003-05-27
Fox, David T. (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
Involving particle-mediated transfecion
C800S278000, C800S287000, C800S288000, C800S300000, C435S418000, C435S419000, C435S430000, C435S430100, C435S470000
Reexamination Certificate
active
06570067
ABSTRACT:
TECHNICAL FIELD
This invention relates to the use of recombinant DNA technology for the transformation of plants. More specifically, this invention concerns the techniques and materials necessary for stable transformation of plant cells and the regeneration of fertile whole plants therefrom.
BACKGROUND ART
Much research in plant molecular biology is now directed to the improvement of plant varieties via use of recombinant DNA techniques. Historically, plant breeders used classical genetic techniques to identify, preserve and crossbreed varietal lines having desirable traits. More recently, new plant varieties were induced by chemicals or by radiation treatment to mutate plant cells which were then regenerated using tissue culture techniques. These random and unpredictable approaches have obvious drawbacks. By the use of recombinant DNA technology, specific genes producing specific proteins, such as those imparting insect resistance, may be introduced into a plant to produce a desired variety with a particular trait. It is thus desirable to provide a method for regeneration of plant cells which have been transformed with recombinant DNA.
DISCLOSURE OF THE INVENTION
This invention provides methods for the construction and use of expression vectors which include at least one gene associated with the expression of a desired agronomic trait or the production of a desired protein. It further provides methods for the transformation of plant cells with the expression vector, and methods for regeneration of whole, fertile transformed plants from transformed cells in tissue culture. Finally, this invention provides transformed plants which have been produced according to the foregoing methods.
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Klein Theodore M.
Sanford John C.
Tomes Dwight T.
Weissinger Arthur
Foley & Lardner
Fox David T.
Pioneer Hi-Bred International , Inc.
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