Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – Involving particle-mediated transfecion
Patent
1994-10-06
1999-11-23
Benzion, Gary
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
Involving particle-mediated transfecion
800278, 800288, 8003201, 8003001, 435412, 435424, 435430, 4354301, C12N 1500, C12N 1582, A01H 106, A01H 400
Patent
active
059903872
ABSTRACT:
The invention provides methods for producing a fertile, stably transformed, Zea mays plant. The methods comprise the steps of (a) providing a foreign DNA comprising an expression vector carrying a gene encoding an agronomic trait; (b) providing a maize embryogenic callus, suspension culture, or immature embryo isolated from a plant; (c) introducing the foreign DNA into the embryogenic callus, suspension culture or immature embryo isolated from a plant by one or more microparticle bombardments; and (d) regenerating fertile transgenic Zea mays plant.
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Klein Theodore M.
Sanford John C.
Tomes Dwight T.
Weissinger Arthur
Benzion Gary
Pioneer Hi-Bred International , Inc.
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