Stable transformation of maize cells by electroporation

Details Stable transformation of maize cells by electroporation Stable transformation of maize cells by electroporation

1994-06-08

1995-12-05

Benzion, Gary

Chemistry: molecular biology and microbiology

Spore forming or isolating process

43524049, 43524050, 4351723, 800205, 935 67, 935 85, C12N 504, A01H 400

Patent

active

054728690

ABSTRACT:
The present invention provides a method to increase the susceptibility of cultured Zea mays cells to stable transformation with recombinant DNA via electroporation, by pretreating the Zea mays cells with certain pectin-degrading enzymes, so that the cells retain their ability to regenerate fertile, transgenic Zea mays plants containing the DNA which is also heritable.

REFERENCES:
patent: 4743548 (1988-05-01), Crossway et al.
patent: 5286634 (1994-02-01), Stadler et al.
Lindsey et al. (1990) Physiologia Plantarum vol. 79-: pp. 168-172.
Potrykus I. (1990) IBID 00179, 125.
Potrykus, I, (1990) Biotechnology vol. 535 p. 2.

Affiliated with

Also associated with

Rating

Stable transformation of maize cells by electroporation does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Stable transformation of maize cells by electroporation, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Stable transformation of maize cells by electroporation will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1373582