Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1985-12-06
1991-01-01
Teskin, Robin L.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4351723, 4352402, 435320, 536 27, 935 1, 935 34, 935 41, 935 70, 935 32, C12P 2100, C12N 1500, C12N 500
Patent
active
049817906
ABSTRACT:
This invention describes stable tat.sub.III cell lines. It is disclosed that by transfecting a preselected tat.sub.III cell line with a vector containing a sufficient amount of the HTLV-III LTR responsive to tat.sub.III gene products for trans-activation and an enhancer upstream of the tat.sub.III responsive segment, it is possible to express high levels of the tat.sub.III gene products. By including a preselected heterologous gene on this vector, it is also possible to express high levels of a desired gene product. A substantially pure protein comprising 86 amino acids and having an apparent molecular weight of about 14,000 dalton and exhibiting trans-activating activity is also disclosed. This protein and polypeptides having trans-activating ability, which is also disclosed, can be used to produce high levels of a desired gene product. A method of detecting the presence of HTLV-III/LAV virus in an individual is also disclosed and comprises the step of:
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Goh Wei C.
Haseltine William A.
Rosen Craig A.
Sodroski Joseph G.
Burrous Beth A.
Conlin David G.
Dana Farber Cancer Institute
Eisenstein Ronald I.
Teskin Robin L.
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