Stable substrate-chromogen solutions for enenzyme activity detec

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase

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435 4, 435 25, C12Q 128

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active

058044043

ABSTRACT:
The preparation and use of stable, ready-to-use substrate-chromogen solutions for the detection and quantification of peroxidase or pseudoperoxidase activity are described. The substrate-chromogen solution according to the present invention contains a buffer, preferably imidazole buffer, a polymer, a hydroperoxide substrate, a chromogenic organic electron donor and electron donor of the carbohydrate type as a stabilizer. The substrate-chromogen solution retains its full peroxidatic activity and develops a negligible color during storage.

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