Stable, single-liquid alpha-amylase reagent

Details Stable, single-liquid alpha-amylase reagent Stable, single-liquid alpha-amylase reagent

1992-05-04

1995-01-24

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435188, 435810, C12Q 140, C12N 996

Patent

active

053842456

ABSTRACT:
In a single liquid alpha-amylase reagent composition comprising an aqueous solution of at least one substrate which is hydrolyzed when mixed with a sample of body fluid containing alpha-amylase to yield a detectable label, alpha-glucosidase from Bacillus stearothermophilus is used to cooperate with the alpha-amylase in the formation of the detectable label and the composition being stable against degradation for a least 6 months at 2.degree. to 10.degree. C.

REFERENCES:
patent: 4310625 (1982-01-01), Modrovich
patent: 4550077 (1985-10-01), Woodbridge
patent: 4649108 (1987-03-01), Blair
Back, J., Increased Thermal Stability of Proteins . . . Biochemistry V 18 (23) 1979, pp. 5191-5196.

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