Stable reagent for determining bilirubin in serum and method of

Details Stable reagent for determining bilirubin in serum and method of Stable reagent for determining bilirubin in serum and method of

1989-05-25

1990-10-23

Hill, Jr., Robert J.

Chemistry: analytical and immunological testing

Heterocyclic carbon compound

Hetero-n

436166, 436903, G01N 2178, G01N 3372

Patent

active

049652109

ABSTRACT:
There is provided a bilirubin assay composition and method of its formation. The composition comprises water, EDTA, of water soluble organic sulfate present in a concentration of about 1 g/l to solution saturation, caffeine in a concentration of from 0 to about 100 g/l and a diazonium salt in a concentration of about 0.05 to about 1.5 mM. The solution has a pH of from about 3.5 to about 6.0 and is stable for at least 18 months at 2.degree. to 8.degree. C.

REFERENCES:
patent: 3585004 (1971-06-01), Mast
patent: 3989462 (1976-11-01), Hirsch
patent: 4119401 (1978-10-01), Sansur et al.
patent: 4311483 (1982-01-01), Perry
patent: 4612290 (1986-09-01), Yazawa et al.
Henry et al, "Clinical Chemistry-Principles and Technics", Second Edition, Harper & Row, New York, 1974, pp. 1045-1050.

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