Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1988-01-20
1991-02-05
Kepplinger, Esther L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
435 4, 435 15, 435 26, 435 19, 435 21, 435810, C12Q 140, C12Q 100
Patent
active
049904450
ABSTRACT:
A stable reagent and kinetic assay for .alpha.-amylase is provided. The reagent comprises a substrate for the .alpha.-amylase and various enzymes and cofactors necessary to produce NADH as the end-product of a series of four reactions. The substrate, enzymes, and cofactors are provided in sufficient excess that the .alpha.-amylase from the test sample is the rate-limiting factor. The concentration of .alpha.-amylase in a test sample is determined by measuring the rate of increase in absorbance caused by the production of NADH. The use of the novel cofactor fructose-1,6-diphosphate has been found to impart improved stability to the reagent.
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Oyen Mark T.
Poudrier Sandra M.
Beckman Instruments Inc.
Grant Arnold
Kepplinger Esther L.
May William H.
Scheiner Toni
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