Stable rabbit-mouse hybridomas and secretion products thereof

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Using tissue cell culture to make a protein or polypeptide

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43524027, 4351722, 935 95, 935 96, 935102, 935104, 530387, 436548, C12P 1200, C12N 500, C12N 1500, G01N 3353

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active

049770810

ABSTRACT:
Rabbit-mouse hybridomas are cultured immediately after fusion in a suspension medium supplemented with normal rabbit serum. This results in stable hybridomas and provides a source of intact rabbit monoclonal antibody immunoglobulin of defined specificity. A hybridoma which secretes intact rabbit antibody specific for Group A Streptococcus is exemplified. Diagnostic compositions and test kits containing rabbit monoclonal antibodies produced from such hybridomas are described.

REFERENCES:
Braun et al., Pathog. Streptococci, Proc. Int. Symp., 7th Meeting, Date 1978, pp. 84-85, Editors: Parker, N. J., Chertsey, Engl.
Kuo et al., Mol. Immunol., vol. 22, pp. 351-359, 1985.
Dreher et al., J. Immunol., vol. 130, pp. 442-448, 1985.
Bruno, G. A., New Techniques in Tumor Localization & Radioimmunoassay, pp. 9-15, 1974, John Wiley & Sons, Editors: Croll, Brady, Hindu & Wallner.
Parker, R. C., In Methods of Tissue Culture, Harper & Roe, Publishers, 1966, p. 193.
Reading, Journ. of Immunol. Methods, 53, 261 (1982).

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