Stable pura vectors and uses therefor

Details Stable pura vectors and uses therefor Stable pura vectors and uses therefor

1995-05-24

1999-10-05

Ketter, James

Drug, bio-affecting and body treating compositions

Antigen, epitope, or other immunospecific immunoeffector

Recombinant or stably-transformed bacterium encoding one or...

4242341, 4242571, 4242581, 4242721, 435 693, 4353201, 536 235, 536 237, 5362372, A61K 3902, C12N 1511, C12N 1563

Patent

active

059619830

ABSTRACT:
This invention pertains to a complementation system for the selection and maintenance of expressed genes in bacterial hosts. The invention provides stable vectors which can be selected and maintained by complementation of chromosomal deletion mutations of purA (adenylosuccinate synthetase), obviating the use of antibiotic resistance genes. This system is useful in production organisms during fermentation and in live vaccine bacteria, such as attenuated Salmonella typhi. This system allows for selection of chromosomal integrants and for selection and stable plasmid maintenance in the vaccinated host without application of external selection pressure.

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