Drug – bio-affecting and body treating compositions – Whole live micro-organism – cell – or virus containing – Genetically modified micro-organism – cell – or virus
Patent
1998-03-23
2000-07-18
Guzo, David
Drug, bio-affecting and body treating compositions
Whole live micro-organism, cell, or virus containing
Genetically modified micro-organism, cell, or virus
424 936, 435 691, 435183, A61K 4800, C12P 2100, C12P 2102, A01N 6300
Patent
active
060903791
ABSTRACT:
A method of infecting insects is disclosed; the method utilizes a form of a baculovirus which is highly efficient at establishing infection and is normally destined to become occluded within the polyhedrin or granulin--Pre-occluded Virus (POV). Specifically, the POV as derived from a polyhedrin-minus or granulin-minus (lacking a functional polyhedrin or granulin gene) baculovirus is fed to insect larvae per os resulting in high infection rates. The stabilization and use of the POV form of polyhedrin-minus baculoviruses for recombinant protein production and as an insecticide is also disclosed.
REFERENCES:
patent: 5071748 (1991-12-01), Miller
patent: 5080807 (1992-01-01), Carr et al.
PCT/US/03542, Mount Sinai School of Music, In Vivo Infection of Live Insects With A Recombinant Baculovirus, Prio date: Aug. 5, 88, pp 12.
Pennock et al, Mol & Cell. Biol., Mar. 1984, p 399-406, Strong and Regulated Expression of Escherichia Coli B-Galactosidase in Insect Cells With A Baculovirus Vector.
Smith et al, Jor. of Virology, May 1983, p 584-593, Molecular Engineering of the Autographa California Nuclear Polyhedrosis Virus Genome: Deletion Mutations Within the Polyhedrin Gene.
Carbonell et al., Gene 73 (1988) 409-418, Synthesis of a Gene Coding for an Insect-Specific Scorpion Neurotoxin and Attempts to Express It Using Baculovirus Vectors.
Luckow, V., Monsanto Corp. Res, 1990, pp 1-35, Cloning and Expression of Heterologous Genes in Insect Cells With Baculovirus Vectors.
Hammock et al, Expression and Effects of the Junenile Hormone Sterase in a Baculovirus, Nature, vol. 344, Mar. 1990, p. 458-460.
Maeda, S., Biochem. & Biophys. Res. Commun, vol. 165, No.3 1989, p. 1177-1183, Increased Insecticidal Effect by a Recombinant Baculovirus Carrying A Synthetic Diuretic Hormone Gene.
Maeda et al., Virology 184, 777-780 (1991), Insecticidal Effects of an Insect-Specific Neurotoxin Expressed by a Recombinant Baculo.
O'Reilly, D. et al., 1989, A Baculovirus blocks insect molting by producing ecdysteroid UDP-glucosyl transferase, Science 245:1110-1112.
Tomalski, M. et al., 1991, Insect Paralysis by baculovirus-mediated expression of a mite neurotoxin gene, Nature 352:82-85.
Merryweather, A. et al., 1990, construction of genetically engineered baculovirus insecticides containing the Bacillus thuringiensis subsp. kurstaki HD-73 delta endotoxin, J.Gen. Virol. 71:1534-1544.
Stewart, L. et al, Construction of an improved baculovirus insecticide containing an insect-specific toxin gene, Nature, pp 352:85-88 Jul., 1991.
O'Reilly, D. et al., 1991, Improvement of a Baculovirus pesticide by deletion of the EGT gene, Biotechnology 9:1086-1089.
Wood, H.A. et al, 1991, Genetically engineered baculoviruses as agents for pest control, Ann. Rev. Microbiol. 45: 69-87).
Maeda, S. et al., 1985, Production of human alpha-interferon in silkworm using a baculovirus vector, Nature 315: 592-594.
Granados, R. et al., 1986, In vivo infection and replication of baculoviruses, The Biology of Baculoviruses, p. 90-108.
Bishop, D. et al., 1988, Field trials of genetically-engineered baculovirus insecticides, p. 143-178.
Hamblin, M. et al, 1990, Co-occlusion and persistence of a baculovirus mutant lacking the polyhedrin gene, Appl. & Environ. Microbiol 56:3057-3062.
Federici, B., 1986, The Biology of Baculoviruses, vol. 1, Ultrastructure of Baculoviruses, p. 61-88.
Van Beek, N. et al., 1987, Alkaline liberated baculoviruses particles retained their infectivity per os for neonate lepidopterous larvae, J. Invertebrate. Pathol. 50:339-340.
Hughes, P. et al, 1981, A synchronous peroral technique for bioassay insect viruses, J. Invert. Pathol. 37:154-159.
Hughes, P. et al., 1986, A modified droplet feeding method for rapid assay of Bacillus thuringiensis and baculoviruses in noctuid larvae, J. Invert. Pathol. 48: 187-192.
Determination by Fluorescence Spectroscopy of the Volume Ingested by Neonate Lepidopterouse Larvae, Notes, Jour. of Invertebrate Pathology, 48, 249-251 1986.
Wood, H.A., 1980, Isolation and replication of an occlusion body-deficient mutant of the Autographa californica polyhedrosis virus, Virol. 105:338-344.
Wood, H.A., 1977, An agar overlay plague assay method for Autographa californica nuclear polyhedrosis virus, J. Invert. Pathol. 29:304-307.
Eldridge, et al., Appl. & Enviro. Micro, May 1992, p 1583-1591.
Kawai, et al, Anim. Sci. Tech., (Jpn) 63 (4) : 349-357 (1992) Production of Porcine Growth Hormone in Silkworn, etc.
Van Beek, N. et al., 1986, Determination of the volume ingested by neonate lepidopterous larvae using the fluorescent dye sodium fluorescein, J. Invert. Pathol. 48:249-251.
O'Reilly, et al., Baculovirus Expression Vectors, A Laboratory Manual, W.H. Freeman and Company, NY.
O'Reilly et al., Insect Biochem, Molec. Biol. vol. 22, No. 4 pp 313-320, 1992.
Bonning, B. et al, Insect Biochem, Molec., Bio. vol. 22, No. 5 pp. 453-458, 1992.
Volkman et al., Jnl. of Invertebrate Pathology, 30: 102-103.
Lesnaw, J., Oral Inoculation of Larvae with Recombinant Baculovirus, Invitrogen Corp. Digest, Mar. 1993, vol. 6--Issue 2.
van den Heuvel, 1993,J.F.J.M et al, Jour. of Virol. Methods, 42, pp. 207-216, A simple and efficient procedure for the oral inoculation of Trichoplusia ni larvae with polyhedrin-negative recombinant baculovirus.
van den Heuvel, J.F.J.M. et al, Phytopathology for Sep., issued Aug. 1992, Expression of Discrete Potyvirus Gene Products By A Non-occluded Recombinant Baculovirus Fed to Trichoplusia ni Larvae.
Doane, et al, The Gypsy Moth: Research Toward Integrated Pest Management, U.S. Dept of Agric, 1981, pp. 461-466.
Shieh, T., Industrial Production of Viral Pesticides, Advances in Virus Research, vol. 36, pp. 315-343.
LeBlanc et al, 1991, Effect of Desiccation, pH, Heat & Ultraviolet Irradiation on Viability of Baculovirus penaei, Jol. of Invertebrate Pathology, 57, 277-286.
Davis et al, 1971, The Stability of a Purified Granulosis Virus of the European Cabbageworm, Pieris brasicae, in Dry Deposits of Intact Capsules, Jour. of Invert. Path. 17: 227-233.
Momoyamka et al., 1989, Inactivation of Baculoviral mid-gut Glandnecrosis BMN virus by UV Irradiation, Sunlight Exposure, Heating & Drying, Fish Pathol. 24(2) , 115-118.
Birnbaum, M.J. et al, An Apoptosis-Inhibiting Gene From a Nuclear Polyhedrosis Virus Encoding a Polypeptide With Cys/His Sequence Motifs, Jnl. of Virology, Apr. 1994, pp. 2521-2528.
Murphy, F.A. et al, Virus Taxonomy, Archives of Virology, Supplement 10, Baculoviridae, pp. 104-113.
Smith, G.E. et al, DNA Homology Among Subgroup A,B, and C Baculoviruses, Virology 123, 1982, pp. 393-406.
Jehle, J.A. et al, The Granulin Gene Region of Cryptophlebia Leucotreta Granulosis Virus: Sequence Analysis and Phylogenetic Considerations, Jnl. of General Virology (1994), 75, pp. 3667-3671.
Crook, N.E. et al, Studies on the Genome Organization of CpGV Abstract only (W41-7), 1995.
Wood, H.A. et al., Per Os Infectivity of Preoccluded Virions from Polyhedrin-Minus Recombinant Baculoviruses, Jor of Invertebrate Pathology, 1993, 62: 4 pages.
Crook, Norman E., et al., Genome Characterization and Genetic Engineering of Cydia pomonella Granulosis Virus, Sep. 1, 1996, Universidad de Cordoba.
Winstanley et al., J. Gen. Virol., vol. 74, pp. 1599-1609, 1993.
Hashimoto et al., J. Gen. Virol., vol. 77, pp. 555-563, 1996.
Tanada et al., "Atlas of Invertebrate Viruses", Ed. Adams and Bonami, CRC Press, 1991, pp. 250-251, 1991.
Winstanley, D., 1996, Recombinant granulovsis viruses for improved biocontrol, Annual Report 1995-96, Horticulture Research International, p52-53.
Boyce Thompson Institute for Plant Research Inc.
Guzo David
LandOfFree
Stable pre-occluded virus particle for use in recombinant protei does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Stable pre-occluded virus particle for use in recombinant protei, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Stable pre-occluded virus particle for use in recombinant protei will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2033609