Stable PQQ-dependent glucose dehydrogenase composition

Details Stable PQQ-dependent glucose dehydrogenase composition Stable PQQ-dependent glucose dehydrogenase composition

2005-04-26

2005-04-26

Marx, Irene (Department: 1651)

Drug, bio-affecting and body treating compositions

Enzyme or coenzyme containing

Oxidoreductases

C424S094300, C435S189000, C435S190000, C435S191000, C435S192000

Reexamination Certificate

active

06884416

ABSTRACT:
The present invention provides a stable lyophilized PQQ-dependent glucose dehydrogenase composition comprising a PQQ-dependent glucose dehydrogenase together with (i) at least one compound selected from the group consisting of aspartic acid, glutamic acid, α-ketoglutaric acid, malic acid, α-ketogluconic acid, α-cyclodextrin and their salts and (ii) an albumin.

REFERENCES:
patent: 09-140378 (1997-06-01), None
Kilty et al., “Reconstitution of Glucose Dehydrogenases Using Synthetic Methoxatin,”Archives of Biochemistry and Biophysics, 218(2), 623-625 (1982).
Geiger et al., “Reversible Thermal Inactivation of the Quinoprotein Glucose Dehydrogenase fromAcinetobacter calcoaceticus,” Biochem. J., 261, 415-421 (1989).
Sode et al., “Preparation of Lyophilized Pyrroloquinoline Quinone Glucose Dehydrogenase Using Trehalose as an Additive,”Biotechnology Techniques, 11(8), 577-580 (Aug. 1997).
Ameyama, “Enzymic Microdetermination of D-Glucose, D-Fructose, D-Gluconate,2-Keto-D-Gluconate, Aldehyde, and Alcohol with Membrane-Bound Dehydrogenases,”Methods in Enzymology, 89,20-29 (1982).

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