Stable mutants of D-N-.alpha.-carbamoylase and process for prepa

Details Stable mutants of D-N-.alpha.-carbamoylase and process for prepa Stable mutants of D-N-.alpha.-carbamoylase and process for prepa

1995-04-03

1999-02-09

Carlson, Karen C.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing alpha or beta amino acid or substituted amino acid...

435 78, 435195, C12P 1304, C12P 1956, C12N 914

Patent

active

058692983

ABSTRACT:
The present invention relates to mutants of D-N-.alpha.-carbamylase wherein at least one of the cysteines in position 243, 250 and 279 of the amino acid sequence of the wild type enzyme is substituted with a different residue selected from natural amino acids; a recombinant plasmid comprising a nucleotidic sequence which encodes for at least one of the mutants of D-N-.alpha.-carbamylase, host microorganisms transformed with said plasmid and a process for the preparation of these mutants by the culture of said microorganisms. The mutants of D-N-.alpha.-carbamylase have a higher enzymatic stability than that of the wild type enzyme and are particularly useful in the preparation of D-.alpha.-amino acids providing an improvement in the production yields.

REFERENCES:
Buson et al. 1996 FEMS Microbiol. Lett 145:55-62.
Grifantini et al 1996 J. Biol. Chem. 271:9326-9331.
Ogawa et al 1994 J Biotech 38:11-19, p. 11 only.
Kim et al 1995 Enz & Microbiol Technol 17:63-67 p. 63 only.

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