Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1997-08-07
1999-09-07
Fonda, Kathleen K.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
4352877, 436169, 549220, C12Q 142, G01N 3122, G01N 2178, C07F 906
Patent
active
059486317
ABSTRACT:
The subject matter of the invention is a stable mixture for the detection of alkaline phosphatase, wherein it contains the N-methylglucamine salt of o-cresolphthalein monophosphoric acid and N-methylglucamine as well as an analytical element for the detection of alkaline phosphatase with a matrix that carries this mixture. In addition the invention concerns a method for the determination of alkaline phosphatase using a salt of o-cresolphthalein monophosphoric acid, wherein the sample is brought into contact with a mixture according to the invention or an analytical element according to the invention and a change in color is observed in the presence of alkaline phosphatase.
A subject matter of the invention is also a salt of o-cresolphthalein monophosphoric acid with N-methylglucamine as well as a process for producing such a salt.
REFERENCES:
patent: 3975405 (1976-08-01), Hamill
patent: 4900665 (1990-02-01), Terashima et al.
patent: 4985414 (1991-01-01), Guethlein et al.
patent: 5211914 (1993-05-01), Vogel et al.
Bossert-Reuther Steffen
Nagel Rolf
Zeibig Thomas
Fonda Kathleen K.
Moran Marjorie A.
Roche Diagnostics GmbH
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