Stable meterologous propagation of CFTR protein variant cDNA

Details Stable meterologous propagation of CFTR protein variant cDNA Stable meterologous propagation of CFTR protein variant cDNA

1996-02-21

1999-01-26

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Using a micro-organism to make a protein or polypeptide

435 691, 435 701, 435 711, 4351723, 435 914, 4353201, 4352523, 435366, 935 23, 935 71, 935 72, 935 70, C12P 2104, C12P 2106, C12N 1509, C12N 1563

Patent

active

058637702

ABSTRACT:
A modified DNA sequence encoding full length cystic fibrosis transmembrane conductance regulator protein is provided to facilitate propagation and/or expression of the protein in living cells and in particular, bacterial cells. The modified DNA sequence comprises at least one of the 13 base pair repeat of exon 6b of the normal gene encoding the conductance regulator protein, as one or more normal nucleotides of the 13 base repeat substituted with an alternate nucleotide which, however, continues to code for the corresponding normal amino acid. Mammalian cells transferred with a vector containing the modified DNA sequence enhances chlorine conductance through the cell wall

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