Stable mammalian cell line expressing a bacteriophage RNA polyme

Details Stable mammalian cell line expressing a bacteriophage RNA polyme Stable mammalian cell line expressing a bacteriophage RNA polyme

1990-03-01

1992-06-30

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4352402, 935 34, 935 60, 935 70, C12N 1500, C12N 510, C12P 2100

Patent

active

051262517

ABSTRACT:
The present invention relates to a eukaryotic cell line which expresses a foreign RNA polymerase gene. The invention further relates to a method of expressing a foreign protein in a eukaryotic environment utilizing the cell line. The present invention allows for the expression of a foreign protein in a eukaryotic cell without requiring transfecting or infecting the cell with a vector carrying the RNA polymerase gene.

REFERENCES:
Fundamental Virology, Second Edition, Raven Press, New York (1991), pp. 410, 776, & 777.
Lieber et al. High level game expression in mammalian cells by a nuclear T7-phage RNA polymerase,Nucleic Acids Research vol. 17, pp. 8485-8493 (1989).
Zhou et al., Synthesis of Functional mRNA in Mammalian Cells by Bacteriophage T3 RNA Polymerase, Molecular and Cellular Biology, Sep. 1990, vol. 10, pp. 4529-4537.
Elroy-Stein et al. Cytoplasmic expression system based on constitutive synthesis of bacteriophage T7 RNA polymerase in mammalian cells, Proc. Natl. Acad. Sci. USA vol. 87, pp. 6743-6747, Sep. 1990 Biochemistry.

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