Stable liquid enzyme concentrate and process for its production

Details Stable liquid enzyme concentrate and process for its production Stable liquid enzyme concentrate and process for its production

1988-12-12

1991-04-23

Lee, Lester L.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435 99, 435188, 435832, C12N 928

Patent

active

050100084

ABSTRACT:
A process for preparing a stable liquid enzyme concentrate containing the alpha-amylase from Bacillus stearothermophilus. A commercial alpha-amylase enzyme preparation is treated with granular starch to absorb the enzyme, and the starch containing the enzyme is separated and then heated in a buffer solution containing calcium ion. The resulting solution, separated from insoluble solid, is then concentrated to give the final product.

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patent: 4578352 (1986-03-01), Katkocin et al.
patent: 4613570 (1986-09-01), Zeman
Pfueller et al., J. Biol. Chem., 244, 48-54 (1969).
Brumm et al., Food Biotechnology, 2(1), 67-80 (1988).

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