Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Reexamination Certificate
2006-10-24
2006-10-24
Gitomer, Ralph (Department: 1655)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
C435S024000, C435S068100
Reexamination Certificate
active
07125685
ABSTRACT:
Proteolytic peptide mass mapping as measured by mass spectrometry provides an important method for the identification of proteins, which are usually identified by matching the measured and calculated m/z values of the proteolytic peptides. A unique identification is, however, heavily dependent upon the mass accuracy and sequence coverage of the fragment ions generated by peptide ionization. The present invention describes a method for increasing the specificity, accuracy and efficiency of the assignments of particular proteolytic peptides and consequent protein identification, by the incorporation of selected amino acid residue(s) enriched with stable isotope(s) into the protein sequence without the need for ultrahigh instrumental accuracy. Selected amino acid(s) are labeled with13C/15N/2H and incorporated into proteins in a sequence-specific manner during cell culturing. Each of these labeled amino acids carries a defined mass change encoded in its monoisotopic distribution pattern. Through their characteristic patterns, the peptides with mass tag(s) can then be readily distinguished from other peptides in mass spectra. The present method of identifying unique proteins can also be extended to protein complexes and will significantly increase data search specificity, efficiency and accuracy for protein identifications.
REFERENCES:
patent: 6653076 (2003-11-01), Franza et al.
patent: 2002/0076817 (2002-06-01), Figeys et al.
patent: 2003/0044864 (2003-03-01), Short et al.
patent: 2004/0072251 (2004-04-01), Anderson
patent: 2005/0164326 (2005-07-01), Figeys et al.
Waugh D. et al. Genetic Tools for Selective Labeling of Proteins with Alpha 15 N Amino Acids. J of Biomolecular NMR. vol. 8, pp. 184-192, 1996.
Veenstra T. et al. Proteome Analysis Using Selective Incorporation of Isotopically Labeled Amino Acids. J American Soc for Mass Spec. Elsevier Science Inc. 11(1)78-82, 2000.
Cottrell Bruce H.
Gitomer Ralph
Los Alamos,National Security, LLC
Santandrea Robert P.
LandOfFree
Stable isotope, site-specific mass tagging for protein... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Stable isotope, site-specific mass tagging for protein..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Stable isotope, site-specific mass tagging for protein... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3709176