Stable high copy number plasmids

Details Stable high copy number plasmids Stable high copy number plasmids

1983-02-17

1986-12-23

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

4351723, 435317, 935 29, 935 42, C12P 2100, C12N 1500, C12N 100

Patent

active

046312579

ABSTRACT:
DNA plasmids are described which are selected mutants in which an altered repressor gene leads to high copy number replication. Elements in the plasmids are modified in such a way that readthrough expression of heterologous DNA inserted in the plasmid will not continue into the replication primer strand. Deletions resulting from interference with replication primer strand transcription are thereby avoided.

REFERENCES:
patent: 4237224 (1980-12-01), Cohen
Shepard et al., " Cell", vol. 18, pp. 267-275, Oct. 1979.
Felipe Cabello, et al., Replication Control in a Composite Plasmid Constructed by in vitro Linkage of Two Distinct Replica, "Nature", 259:285-290 (1976).
Barry Polisky, et al., A Plasmid Cloning Vehicle Allowing Regulated Expression of Eukaryotic DNA in Bacteria, "PNAS", USA 73:3900-3904 (1976).
Reiner Gentz, et al., Cloning and Analysis of Strong Promoters is Made Possible by the Downstream Placement of a RNA Termination Signal, "PNAS", USA 78:4936-4940 (1981).
E. Beck, et al., Nucleotide Sequence of Bacteriophage of DNA, "Nucleic Acids Research", vol. 5, No. 12, pp. 4495-4503 (1978).

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