Stable gene amplification in chromosomal DNA of prokaryotic micr

Details Stable gene amplification in chromosomal DNA of prokaryotic micr Stable gene amplification in chromosomal DNA of prokaryotic micr

1998-03-27

2000-09-26

Guzo, David

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 691, 4353201, 4352523, 43525231, 43525233, 43525234, 43525235, 435212, 435219, 435222, C12Q 168, C12N 121, C12P 2102

Patent

active

061240974

ABSTRACT:
Transformed prokaryotic hosts are provided comprising two or more copies of a DNA sequence stably maintained in their chromosome, said DNA sequence comprising a gene encoding a polypeptide of interest wherein said copies are separated by endogenous chromosomal DNA sequences. Methods are also provided for producing said transformed host strains. The transformed host strains are capable of increased production of the polypeptide of interest compared to host strains which already produce said polypeptide. Preferred host strains are Bacillus novo species PB92 which produces a high-alkaline proteolytic enzyme and B. licheniformis strain T5 which produces a thermostable .alpha.-amylase, and mutants and variants of said strains. Preferred poly-peptide encoding genes are the protease encoding gene obtainable from Bacillus PB92 and the .alpha.-amylase encoding gene obtainable from B. licheniformis strain T5.

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