Stable encapsulated reference nucleic acid and method of making

Details Stable encapsulated reference nucleic acid and method of making Stable encapsulated reference nucleic acid and method of making

1997-07-31

2000-06-13

Marschel, Ardin H.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 5, 435 4, 435 72, 435 29, 435 405, 4351731, 435174, 435325, 435245, 4353071, 514 44, 536 253, 935 77, C12Q 168

Patent

active

060748257

ABSTRACT:
Stable reference nucleic acid for use in all steps of molecular screening and diagnostic assays and method of making. A desired nucleic acid sequence is amplified, ligated into an expression vector, and used to transform a vehicle. A cellular vehicle is subsequently killed without affecting the encapsulated nucleic acid. The vehicle membrane is stabilized under controlled conditions to a stability substantially matching that of a test cell membrane. The recovered nucleic acid is used as a standard or control in molecular diagnostic and genetic testing assays.

REFERENCES:
patent: 5550050 (1996-08-01), Holland et al.
patent: 5665383 (1997-09-01), Grinstaff et al.
patent: 5672345 (1997-09-01), Curtiss, III
patent: 5677124 (1997-10-01), DuBois et al.
Rosenstraus, Maurice et al., An Internal Control for Routine Diagnostic PCR: Design, Properties, and Effect on Clinical Performance, Journal of Clinical Microbiology; Jan. 1998. vol. 36, No. J, pp. 191-197.
Pasloske, B.I. et al., "Novel ribonuclease resistant RNA standards for HIV diagnostics." Clin. Chem. vol. 42, pp. 1890-1891, 1996.

Affiliated with

Also associated with

Rating

Stable encapsulated reference nucleic acid and method of making does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Stable encapsulated reference nucleic acid and method of making, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Stable encapsulated reference nucleic acid and method of making will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2067441