Stable encapsulated reference nucleic acid and method of making

Details Stable encapsulated reference nucleic acid and method of making Stable encapsulated reference nucleic acid and method of making

1997-12-23

1999-11-30

Marschel, Ardin H.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 5, 435440, 536 221, 536 231, 514 1, 514 44, C12Q 170, C12Q 168, C07H 1900, C07H 2102

Patent

active

059940786

ABSTRACT:
Stable reference nucleic acid for use in all steps of molecular screening and diagnostic assays and method of making. A desired nucleic acid sequence is amplified, ligated into an expression vector, and used to transform a vehicle. A cellular vehicle is subsequently killed without affecting the encapsulated nucleic acid. The vehicle membrane is stabilized under controlled conditions to a stability substantially matching that of a test cell membrane. The recovered nucleic acid is used as a standard or control in molecular diagnostic and genetic testing assays.

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