Stable DNA constructs

Details Stable DNA constructs Stable DNA constructs

1995-06-06

1997-12-23

Ketter, James

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 691, 435 711, 435171, 4351723, 4352542, 43525421, 43525521, C12P 2102

Patent

active

057006431

ABSTRACT:
Methods are provided for producing protein products in host cells and for selecting transformed cells comprising the step of transforming the host cell with a DNA molecule comprising a gene which complements a deficiency in the host cell. The host cell is a strain having a deficiency in a function necessary for normal cell growth. The gene in the DNA molecule, such as a plasmid, which complements the deficiency serves as a selection marker whereby the growth conditions for selection may comprise a conventional complex medium.

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