Stable DNA constructs

Details Stable DNA constructs Stable DNA constructs

1993-10-12

1996-06-18

Fleisher, Mindy

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 691, 43525421, 4353201, C12Q 168, C12P 2102, C12N 119, C12N 1581

Patent

active

055276687

ABSTRACT:
Methods are provided for producing protein products in host cells and for selecting transformed cells comprising the step of transforming the host cell with a DNA molecule comprising a gene which complements a deficiency in the host cell. The host cell is a strain having a deficiency in a function necessary for normal cell growth. The gene in the DNA molecule, such as a plasmid, which complements the deficiency serves as a selection marker whereby the growth conditions for selection may comprise a conventional complex medium.

REFERENCES:
patent: 4237224 (1980-12-01), Cohen et al.
patent: 4506013 (1985-03-01), Hershberger et al.
patent: 4931373 (1990-06-01), Kawasaki et al.
Russell et al J. Biol. Chem. 258 pp. 143-144 1983.
Erhart et al. J. Bact. 156(2) pp. 625-635 1983.
Kuo et al. 1983 Mol Cell Biol. vol. 3, pp. 1730-1737, 1983.
Nasmyth et al. 1980, Proc. Natl. Acad. Sci., vol. 77, (4) pp. 2119-2123.
Alber et al. 1982 J Mol Gen. vol. 1, pp. 419-434.
Carter et al., 1983, Yeast Genetics, Spencer et al. Editors, Springer-Verlag pp. 1-28.
Arendes et al. 1983 Proc. Natl. Acad Sci V80 673-677.
Walsh et al 1983 J. Bact V154 1002-1009.

Affiliated with

Also associated with

Rating

Stable DNA constructs does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Stable DNA constructs, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Stable DNA constructs will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-222065