Stable chromogenic substrate mixture of indoxyl phosphate and te

Details Stable chromogenic substrate mixture of indoxyl phosphate and te Stable chromogenic substrate mixture of indoxyl phosphate and te

1988-12-30

1990-08-07

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 26, 435 29, 435810, 435 4, 436164, C12Q 142

Patent

active

049467760

ABSTRACT:
A stable chromogenic substrate mixture of indoxyl phosphate and tetrazolium salt is prepared by dissolving the phosphate in DMF and combining the same with Tris buffer containing magnesium chloride and followed by combination of the same with a DMF solution of tetrazolium salt. The resulting mixture is stored below room temperature in the absence of light.

REFERENCES:
patent: 4613569 (1986-09-01), Geiser et al.
patent: 4748115 (1988-05-01), Steaffens

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