Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1992-07-20
1994-01-11
Griffin, Ronald W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435 4, 435 6, 435810, 435975, 435 15, 436 17, C12Q 132
Patent
active
052780449
ABSTRACT:
An aqueous coenzyme reagent composition contains NADH, a carbonate/bicarbonate buffer (with a pH of about 9.5-11) and water. Both NADH and the buffer are at low concentrations, e.g., about 2-5 mM for NADH and about 2-15 mM for carbonate/bicarbonate. When this coenzyme reagent is mixed with an enzyme reagent, the mixture achieves a neutral pH. The combination of high pH, low NADH concentration and low buffer concentration permit the aqueous reagent to be stored for extended periods at low temperatures without NADH degrading to form impurities which interfere with or inhibit enzyme activity, such as the activity of lactate dehydrogenase or malate dehydrogenase in an assay for ALT or AST.
REFERENCES:
patent: 4153511 (1979-05-01), Modrovich
patent: 4271264 (1981-06-01), Modrovich
patent: 4277562 (1981-07-01), Modrovich
patent: 4310624 (1982-01-01), Modrovich
patent: 4372874 (1983-02-01), Modrovich
patent: 4394449 (1983-07-01), Modrovich
W. Gerhardt et al, "Quality Control of NADH" Scand. J. of Clin & Lab Invest., vol. 33, pp. 1-51 (1974).
S. A. Margolis et al, "Purification & Analysis of the Purity of NADH", Clin. Chem., vol. 22, No. 8, pp. 1322-1329(1976).
D. Northrup et al, "Purification of Reduced Nicotinamide . . . " Methods In Enzymology, vol. 122, pp. 152-154 (1986).
Beckman Instruments Inc., "Beckman Liquid-Stat (R) Reagent AST" (1987).
Beckman Instruments Inc., "Beckman Liquid-Stat (R) Reagent ALT" (1987).
Chem. Abstract 104:84922d (1986).
Chem Abstr 84:175661c (1976).
Adiletto Carol A.
San George Richard C.
Griffin Ronald W.
Instrumentation Laboratory S.p.A.
Webber Pamela S.
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