Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1991-05-30
1993-07-06
Rosen, Sam
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 792, 435 793, 435 18, 435 19, C12Q 142
Patent
active
052253287
ABSTRACT:
Methods and compositions for membrane flow-through assays using substrate and enzyme conjugate is described. A preferred method of the present invention comprises providing E. coli alkaline phosphatase (ECAP) conjugate, with additional color enhancement provided by combining lithium salt with the substrate, calcium and/or magnesium salts with the conjugate, and polyalcohol polymers with the conjugate. Color development is further enhanced by spotting tetrazolium dyes, such as the Nitro Blue tetrazolium series, onto the membrane.
REFERENCES:
patent: 4659657 (1987-04-01), Harnisch
patent: 5035998 (1991-07-01), Eltz et al.
patent: 5077198 (1991-12-01), Shih et al.
Quidel Corporation
Rosen Sam
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