Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2007-02-13
2007-02-13
Guzo, David (Department: 1636)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C435S320100, C435S455000
Reexamination Certificate
active
11155446
ABSTRACT:
The disclosure concerns stable expression systems for production of a protein comprising: a) a species ofAcanthamoebaas a host organism; b) a DNA vector which comprises a selection marker gene which codes for a protein which, after transformation of the host organism, allows selection of positive transformants, where expression of the selection marker gene is controlled by at least one genetic regulatory element which is homologous in the host organism; and c) a DNA vector which comprises a heterologous gene for the expression of a heterologous protein, where the expression of the protein is controlled by at least one genetic regulatory element which is homologous to the host organism.
REFERENCES:
Kong, et al., “Intracellular Localization and Dynamics of Myosin-II and Myosin-IC in LiveAcanthamoebaby Transient Transfection of EGFP Fusion Proteins”, Journal of Cell Science 115(24):4993-5002 (2002).
Lefebre, et al., “Construction and Evaluation of Plasmid Vectors Optimized for Constitutive and Regulated Gene Expression in Burkholderia Cepacia Complex Isolates”, Applied and Environmental Microbiology 68(12):5956-5964 (2002).
Yin, et al., “Stable Transfection ofAcanthamoeba”, Candian Journal of Microbiology, 43:239-244 (1997).
Hu et al., “AnAcanthamoeba polyubiquitinGene and Application of its Promoter to the Establishment of a Transient Transfection System” 1351:126-136 (1997).
Butler, Esq. Gregory B.
Guzo David
The University of Vermont and State Agriculture College
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