Stabilized thermostable nucleic acid polymerase compositions con

Details Stabilized thermostable nucleic acid polymerase compositions con Stabilized thermostable nucleic acid polymerase compositions con

1992-04-24

2000-10-03

Naff, David M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Stablizing an enzyme by forming a mixture, an adduct or a...

435 6, 435 691, 435 911, 435 912, 183471, 183478, 183488, 183814, C12N 996, C12N 900, C12P 1934, C12P 2106

Patent

active

061271551

ABSTRACT:
A purified thermostable nucleic acid polymerase is obtained that has unique characteristics. Preferably the nucleic acid polymerase is DNA polymerase isolated from a Thermus aquaticus species and has a molecular weight of about 86,000-95,000 daltons. The thermostable nucleic acid polymerase may be native or recombinant and may be used in a temperature-cycling chain reaction wherein at least one nucleic acid sequence is amplified in quantity from an existing sequence with the aid of selected primers and nucleotide triphosphates. The nucleic acid polymerase is preferably stored in a buffer containing non-ionic detergents that lends stability to the nucleic acid polymerase. A preferred buffer contains glycerol, polyoxyethylated sorbitan monolaurate, ethoxylated nonyl phenol and gelatin.

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