Stabilized substrate for use in an immunoassay

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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Details

435 792, 435 21, 435968, 435963, G01N 3352, C12Q 142

Patent

active

051438259

ABSTRACT:
A 4-methylumbelliferyl phosphate substrate for use in conducting an immunoassay is disclosed. The substrate contains 0.1 gram mole per liter 2-amino-2-methyl-1-propanol, 0.01 to 0.05 gram mole per liter EDTA, added as trisodium EDTA, 1 gram per liter sodium azide, 1.2 milligram mole per liter 4-methylumbelliferyl phosphate and 4 milligram moles per liter tetramisole. The pH of the substrate disclosed is 10.3.

REFERENCES:
patent: 4400464 (1983-08-01), Vormbrock et al.
patent: 4666830 (1987-05-01), Wagner
Grant & Hackh's Chemical Dictionary, Grant et al, Editors 5th Ed. 1987 p. 200.

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