Stabilized proteolytic solution and reagent kit

Details Stabilized proteolytic solution and reagent kit Stabilized proteolytic solution and reagent kit

1990-12-18

1993-03-09

Brown, Johnnie R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 72, 435 725, 435810, 435 23, 435212, 435188, C12Q 100

Patent

active

051926571

ABSTRACT:
A stabilized proteolytic solution has been developed which can be used to treat selected cells, especially red blood cells. A preferred stabilized solution of the invention comprises about 0.1% to about 0.3% ficin in a citrate-buffered saline solution containing mannitol, L-cysteine, dithiothrietol in an active concentration of less than about 10 mM, and EDTA. A kit for qualitatively identifying unexpected blood group antibodies comprising a series of untreated and ficin-treated red blood cells, a stabilized ficin reagent, a red blood cell diluent and an enzyme control is also provided.

REFERENCES:
patent: 4275053 (1981-06-01), Rosenfield et al.
patent: 4403037 (1983-09-01), Coates
patent: 4608246 (1986-08-01), Bayer et al.
S. D. Rolih, Journal of Blood Group Serology and Education, vol. 2, No. 6 1986, by The American Red Cross, pp. 1 and 106-110.
Leiner and Friedenson, Methods in Enzymology, vol. XIX, Proteolytic Enzymes, 1970, pp. 260-273.
Klibanov, Biochemical Society Transactions, 600th Meeting, Oxford, vol. 11, pp. 19-21.
Branch and Petz, American Society of Clinical Pathologists, Aug. 1982, vol. 78, No. 2, pp. 161-167.

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