Stabilized polyacrylamide gels and system for SDS electrophoresi

Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge

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204299R, C25B 700

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active

044810946

ABSTRACT:
An improved polyacrylamide gel in a gel buffer, a system and method for conducting discontinuous sodium dodecyl sulfate electrophoresis is disclosed. The improved gel involves the use of a salt of 2-amino-2-methyl-1,3-propanediol at a pH of from 6.4 to 7.3 The improved process involves the use of the gel in combination with 2-amino-2-methyl-1,3-propanediol taurine as an electrolyte buffer at a pH of about 8.0 to 10.0.

REFERENCES:
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patent: 3948743 (1976-04-01), Monthoney et al.
patent: 4246084 (1981-01-01), Gurske
patent: 4306956 (1981-12-01), de Castro et al.
Weber et al., J. of Biol. Chem., 244:4406, (1969), "The Reliability of Molecular Weight Determinations by SDS-Page".
Tamura et al., J. Biochem., 71(3):543, (1972), "A New Buffer System for Disc Electrophoresis . . . Proteins".
Wyckoff et al., Anal. Biochem., 78:459, (1977), "Polyacrylamide Gel Electrophoresis in SDS-. . . Procedure".
Catsimpoolas, Ed., Methods of Protein Separation, vol. 2, (1976).

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