Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...
Patent
1997-03-17
1999-06-29
Reeves, Julie
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Blood proteins or globulins, e.g., proteoglycans, platelet...
5303877, 5303905, 4241331, 4241351, 435 696, 435 697, 435328, A61K 39395, C07K 1600
Patent
active
059170212
ABSTRACT:
The present invention relates to a stabilized protein composition comprising a monomeric protein and a storage-stabilizing amount of sucrose, histidine or glycine, which is sufficient to inhibit aggregation of the protein molecules during freeze/thaw cycles, and methods therefor. More particularly, this invention relates to pharmaceutically-acceptable, single-chain antigen-binding protein compositions having increased frozen-storage stability, especially to freeze/thaw cycles.
REFERENCES:
patent: 3950513 (1976-04-01), Jenson
patent: 4496537 (1985-01-01), Kwan
patent: 4597966 (1986-07-01), Zolton et al.
patent: 4645830 (1987-02-01), Yasushi et al.
patent: 4704692 (1987-11-01), Ladner
patent: 4777043 (1988-10-01), Bennett et al.
patent: 4806524 (1989-02-01), Kawaguchi et al.
patent: 4946778 (1990-08-01), Ladner et al.
patent: 5096885 (1992-03-01), Pearlman et al.
patent: 5260203 (1993-11-01), Ladner et al.
patent: 5358708 (1994-10-01), Patel
patent: 5591828 (1997-01-01), Boslet et al.
patent: 5644030 (1997-07-01), Faulmann
Adams, G.P., et al., "Highly Specific in Vivo Tumor Targeting by Monovalent and Divalent Forms of 741F8 Anti-c-erbB-2 Single-Chain Fv," Cancer Res. 53:4026-4034 (1993).
Buchner, J., and Rudolph, R., "Renaturation, Purification and Characterization of Recombinant F.sub.ab -Fragments Produced in Escherichia coli," Bio/Technology 9:157-162 (1991).
Buchner, J., et al., "A Method for Increasing the Yield of Properly Folded Recombinant Fusion Proteins: Single-Chain Immunotoxins from Renaturation of Bacterial Inclusion Bodies," Anal. Biochem. 205:263-270 (1992).
Colcher, D., et al., "In Vivo Tumor Targeting of a Recombinant Single-Chain Antigen-Binding Protein," J. Natl. Cancer Inst. 82:1191-1197 (1990).
Colombo, M.F., et al., "Protein Solvation in Allosteric Regulation: A Water Effect on Hemoglobin," Science 256:655-659 (1992).
Crowe, J.H., et al., "Stabilization of dry phospholipid bilayers and proteins by sugars," Biochem. J. 242:1-10 (1987).
Holliger, P., et al., ""Diabodies": Small bivalent and bispecific antibody fragments," Proc. Natl. Acad. Sci. USA 90:6444-6448 (1993).
Lee, L. Stanford, "Protein Folding and Polymerization Study with a Recombinant Single Chain Antibody," FASEB J. 9:A410 (Abstract 2377) (Mar. 1995).
Levine, R.L., "Oxidative Modification of Glutamine Synthetase. II. Characterization of the Ascorbate Model System," J. Biol. Chem. 258:11828-11833 (1983).
Mezes, P.S., et al., "Molecular Design of Anti-Tumor Single Chain Fv Species," Third Annual IBC International Conference on Antibody Engineering, San Diego, CA (1992) (one page).
Milenic, D.E., et al., "Construction, Binding Properties, Metabolism, and Tumor Targeting of a Single-Chain Fv Derived from the Pancarcinoma Monoclonal Antibody CC49," Cancer Res. 51:6363-6371 (1991).
Muller, J.D., and Nienhaus, G.U., "Ligand Binding to Anti-Fluorescyl Antibodies: Stability of the Antigen Binding Site," Biochemistry 33:6221-6227 (May 1994).
Newton, D.L., et al., "Expression and Characterization of Recombinant Human Eosinophil-derived Neurotoxin and Eosinophil-derived Neurotoxin-Anti-transferrin Receptor sFv," J. Biol. Chem. 269:26739-26745 (Oct. 1994).
Pantoliano, M.W., et al., "Conformational Stability, Folding, and Ligand-Binding Affinity of Single-Chain Fv Immunoglobulin Fragments Expressed in Escherichia coli," Biochemistry 30:10117-10125 (1991).
Rand, R.P., "Raising Water to New Heights," Science 256:618 (1992).
Sawyer, J.R., et al., "The effects of induction conditions on production of a soluble anti-tumor sFv in Escherichia coli," Protein Engineering 7:1401-1406 (Nov. 1994).
Wang, Y.J., and Hanson, M.A., "Parenteral Formulations of Proteins and Peptides: Stability and Stabilizers," J. Parenteral Sci. and Technol. 42(Suppl.):S4-S26 (1988).
Weidner, K.M., et al., "Molecular Stabilization Effects of Interactions between Anti-metatype Antibodies and Liganded Antibody," J. Biol. Chem. 267:10281-10288 (1992).
Whitlow, M., and Filpula, D., "Single-Chain Fv Proteins and Their Fusion Proteins," Methods 2:97-105 (1991).
Whitlow, M., et al., "An improved linker for single-chain Fv with reduced aggregation and enhanced proteolytic stability," Protein Engineering 6:989-995 (1993).
Whitlow, M., et al., "Multivalent Fvs: characterization of single-chain Fv oligomers and preparation of a bispecific Fv," Protein Engineering 7:1017-1026 (Aug. 1994).
Harlow et al Anitbodies: A laboratory Manual Cold Spring Harbor Laboratory Chapter 12, pp. 498 and 549, 1988.
Webster's II New Riverside University Dictionary, The Riverside Publishing House pp. 120,505, 509, 697, 1199, 1303, 1994.
Enzon Inc.
Reeves Julie
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