Stabilized lyophilized mammalian cells and method of making same

Details Stabilized lyophilized mammalian cells and method of making same Stabilized lyophilized mammalian cells and method of making same

1988-10-20

1991-10-22

Saunders, David A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 1, 435 2, 435 721, 435 723, 435 724, 435 29, 435 34, 4352402, 43524027, 435243, 435260, 436 8, 436 10, 436 63, 436 64, A01N 102, C12Q 168, G01N 33569

Patent

active

050595180

ABSTRACT:
A method of lyophilizing mammalian cells to produce preserved human cells, hybridoma cell lines, tissue cells, and control cells for immunoassays and other hematological measurements. Prior to freezing and lyophilizing, the prepared mammalian cell pellet is suspended in a solution of trehalose in an isotonic fluid prepared at a specified optimal concentration and incubated at room temperature for a designated time period. The lyophilized cells when rehydrated, retain their optimal physiological characteristics suitable for use as an analytical control and retain said characteristics after storage at 2.degree.-8.degree. C. for a period in excess of five (5) months.

REFERENCES:
patent: 4649106 (1987-03-01), Schlossman et al.
patent: 4874690 (1989-10-01), Goodrich, Jr. et al.
Moran et al., Science, 198, 149-157, 1977.

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