Stabilized liquid enzyme compositions for diagnostic determinati

Details Stabilized liquid enzyme compositions for diagnostic determinati Stabilized liquid enzyme compositions for diagnostic determinati

1978-04-24

1982-01-12

Naff, David M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

435 14, 435 16, 435 17, 435 26, 435188, 435190, 435194, C12Q 132, C12Q 150, C12Q 152, C12N 996

Patent

active

043106250

ABSTRACT:
Labile enzymes for use in biological diagnostic determinations are stabilized for long terms by forming a solution of the enzyme in an aqueous media containing at least 20% organic solvent such as 30% aqueous propane diol in the presence of a small amount of polymer such as 0.1% gelatin and then diluting the media at least 20 times with water while maintaining the polymer concentration at least 0.01%. The resultant liquid enzyme composition can be stored for extended periods without loss of significant enzyme catalytic activity. Stability is further enhanced by including from 1 to 18% of salts and 0.1% bacteriastatic agents in the liquid enzyme composition. The liquid enzyme composition enzyme can further contain an enzyme substrate and buffer salts.

REFERENCES:
patent: 3557002 (1971-01-01), McCarty
patent: 3627688 (1971-12-01), McCarty et al.
patent: 3764478 (1973-10-01), Bergmeyer et al.
patent: 3819528 (1974-06-01), Berry
George, et al. Stabilization of Lactate and Malete Dehydrogenase by Organic Solvents Biochim. Biophys. Acta. vol. 191, 1969 (pp. 466-468).

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