Stabilized liquid enzyme composition for glucose determination

Details Stabilized liquid enzyme composition for glucose determination Stabilized liquid enzyme composition for glucose determination

1986-07-15

1990-01-30

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving glucose or galactose

435 4, 435 15, 435 25, 435 26, 435 27, 435 28, 435810, C12Q 154, C12Q 148, C12Q 132

Patent

active

048973467

ABSTRACT:
A homogeneous liquid enzyme reagent for the quantitative determination of glucose comprises the enzymes hexokinase and glucose-6-phosphate dehydrogenase in a solution of glycerol (30% v/v) in water, and a stablizer system comprising a heavy metal ion chelating agent. Preferably the stabilizers also comprise an antioxidant and a microbic-control agent. The stabilizers are in sufficient amounts such that the enzyme reagent has a shelf life of at least about two years when stored at a temperature of from 2.degree. to 8.degree. C.

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Flores et al., Clinical Chemistry, vol. 32, No. 6 (1986), p. 1105.
Tietz (ed.), Textbook of Clinical Chemistry, (1986), pp. 784-787.

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